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02501 primescripttm reverse transcriptase takara  (TaKaRa)


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    TaKaRa 02501 primescripttm reverse transcriptase takara
    02501 Primescripttm Reverse Transcriptase Takara, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 89544 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/02501+primescripttm+reverse+transcriptase+takara/PrimeScript+RT+Reagent+Kit/pm41997144-265-110-114
    Average 99 stars, based on 89544 article reviews
    02501 primescripttm reverse transcriptase takara - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Virus:

    Article Title: Tolerance to extracellular acidic pH facilitates tumor plasticity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FAM129C Abcam #ab113755 PIGR Abcam #ab96196; RRID: AB_10677612 β-actin Sigma-Aldrich #A5441; RRID: AB_476744 GPX4 Cell Signaling Technology #52455; RRID: AB_2924984 RIP Cell Signaling Technology #3493; RRID: AB_2305314 phospho-RIP Cell Signaling Technology #65746; RRID: AB_2799693 MLKL Cell Signaling Technology #14993; RRID: AB_2721822 phospho-MLKL Cell Signaling Technology #91689; RRID: AB_2732034 cleaved Caspase-3 Cell Signaling Technology #9661S; RRID: AB_2341188 Caspase-3 Cell Signaling Technology #9662S; RRID: AB_331439 GSDMD Cell Signaling Technology #97558S; RRID: AB_2864253 ZEB1 Cell Signaling Technology #D80D3; RRID: AB_1904164 E-cadherin Cell Signaling Technology #24E10; RRID: AB_2291471 Vimentin Cell Signaling Technology #D21H3; RRID: AB_10695459 Snail Cell Signaling Technology #C15D3; RRID: AB_2255011 C3 Cell Signaling Technology #97425; RRID: AB_2313773 C5a Abcam #202039; RRID: AB_2313773 donkey anti-mouse IgG Sigma-Aldrich #A4416; RRID: AB_2075239 anti-rabbit Cell Signaling Technology #7074P2; RRID: AB_2099233 CD16/32 BioLegend #101301; RRID: AB_312800 CD4 BD Biosciences #565634; RRID: AB_2739312 CD8 BD Biosciences #565968; RRID: AB_2739421 CD11b BioLegend #101227; RRID: AB_393577 I-A/I-E BD Biosciences #745580; RRID: AB_2743096 B220 BD Biosciences #17045282; RRID:AB_469395 Ly6C BioLegend #128007; RRID: AB_1186133 CD11c BD Biosciences #565451; RRID: AB_2744278 NK1.1 BioLegend #156537; RRID: AB_3083138 F4/80 BioLegend #123107; RRID: AB_893500 Ly6G BD Biosciences #746448; RRID: AB_2743751 CD45 BD Biosciences #557659; RRID: AB_396774 APC-conjugated PD-1 BioLegend #135210; RRID: AB_2159183 Brilliant Violet 421-conjugated KLRG1 BioLegend #138413; RRID:AB_10918627 APC-Cy7-conjugated CD44 BioLegend #103027; RRID: AB_830784 PerCP-Cy5.5-conjugated CD62L BioLegend #104431; RRID:AB_2187123 PE-Cy7-conjugated Sca-1 BioLegend #108113; RRID: AB_493597 FITC-conjugated CD122 BioLegend #123207; RRID: AB_940611 anti-rat CD11b BD Biosciences #550282; RRID: AB_393577 anti-hamster CD31 Millipore #MAB1398Z; RRID: AB_94207 rat anti-mouse F4/80 Bio-Rad #MCA497GA; RRID: AB_323806 rat anti-mouse Ly-6G BioLegend #127602; RRID: AB_1089180 Anti-MLKL (phospho S358) antibody Abcam ab187091; RRID: AB_2619685 Alexa Fluor 488-conjugated goat anti-rat Thermo Fisher Scientific #A11006; RRID: AB_2534074 (Continued on next page) Cell Reports ▪▪, 117226, ▪▪, 2026 17 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Alexa Fluor 568-conjugated goat anti-hamster Iwai Chemicals #127-165-160; RRID: AB_2535761 Alexa Fluor 488-conjugated goat anti-rabbit Thermo Fisher Scientific #A11034; RRID: AB_2576217 PerCP-Cy5.5 anti-CD8 BioLegend #100733; RRID: AB_2075239 Alexa Fluor 647 anti-mouse IFN-γ BioLegend #505816; RRID: AB_493315 anti-mouse PD-L1 Selleck #A2115; RRID: AB_3675704 Bacterial and virus strains DH5α Thermo Fisher Scientific #18258012 Chemicals, peptides, and recombinant proteins DMEM (High Glucose) Nacalai Tesque #08459-64 RPMI 1640 Medium Nacalai Tesque #30264-56 Fetal bovine serum Nissui #174012 7-Cl-O-Nec1 Abcam #ab221984 zVAD-FMK BD Biosciences #305664 Necrostatin-1 Enzo Life Sciences #BML-AP309-0020 Ferrostatin-1 MedChemExpress #HY-100579 cOmpleteTM Protease Inhibitor Cocktail (1×) Roche #11697498001 Chemi-Lumi One Ultra Nacalai Tesque #02230-54 Isogen reagent Nippon Gene #311-02501 PrimeScriptTM reverse transcriptase Takara #RR037A THUNDERBIRD® SYBR® qPCR Mix Toyobo #QPS-201 Sepasol®-RNA I Super G Nacalai Tesque #09379-55 Polybrene Sigma-Aldrich #H9268 4% paraformaldehyde FUJIFILM Wako #163-20145 Acetone Nacalai Tesque #00529-85 Puromycin InvivoGen #ant-pr-1 RBC Lysis Buffer Invitrogen #00-4333-57 Dako Protein Block, Serum-free Agilent #X090930-2 DAPI-Fluoromount-G Southern Biotech #0100-20 Dextran, SNARFTM-1, 70,000 MW, Anionic Invitrogen D3304 Pimo-yne Dr. Sando, University of Tokyo N/A PMX53 Selleck #S6293 Critical commercial assays Calcein AM (Ex/Em = 480/515 nm) Thermo Fisher Scientific #C3099 LDH Cytotoxicity Detection Kit Nacalai Tesque #289-97301 MiSeq v2 Reagent Kit Illumina #MS-102-2002 NucleoSpin Tissue Kit Takara #740952.50 Human Complement C3a ELISA Kit Invitrogen BMS2089 Human Complement C5a ELISA Kit Invitrogen BMS2088 KAPA HiFi HotStart ReadyMix KAPA Biosystems #KK2602 FastGene Gel/PCR Extraction Kit Nippon Genetics #FG-91202 Experimental models: Cell lines .. MIA-PaCa2 ATCC CRL-1420 HeLa ATCC CCL-2 HSML Dr. Kudoh, Hirosaki University, Japan N/A HEK293T ATCC CRL-11268 (Continued on next page) 18 Cell Reports ▪▪, 117226, ▪▪, 2026 Article ll OPEN ACCESS

    Recombinant:

    Article Title: Tolerance to extracellular acidic pH facilitates tumor plasticity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FAM129C Abcam #ab113755 PIGR Abcam #ab96196; RRID: AB_10677612 β-actin Sigma-Aldrich #A5441; RRID: AB_476744 GPX4 Cell Signaling Technology #52455; RRID: AB_2924984 RIP Cell Signaling Technology #3493; RRID: AB_2305314 phospho-RIP Cell Signaling Technology #65746; RRID: AB_2799693 MLKL Cell Signaling Technology #14993; RRID: AB_2721822 phospho-MLKL Cell Signaling Technology #91689; RRID: AB_2732034 cleaved Caspase-3 Cell Signaling Technology #9661S; RRID: AB_2341188 Caspase-3 Cell Signaling Technology #9662S; RRID: AB_331439 GSDMD Cell Signaling Technology #97558S; RRID: AB_2864253 ZEB1 Cell Signaling Technology #D80D3; RRID: AB_1904164 E-cadherin Cell Signaling Technology #24E10; RRID: AB_2291471 Vimentin Cell Signaling Technology #D21H3; RRID: AB_10695459 Snail Cell Signaling Technology #C15D3; RRID: AB_2255011 C3 Cell Signaling Technology #97425; RRID: AB_2313773 C5a Abcam #202039; RRID: AB_2313773 donkey anti-mouse IgG Sigma-Aldrich #A4416; RRID: AB_2075239 anti-rabbit Cell Signaling Technology #7074P2; RRID: AB_2099233 CD16/32 BioLegend #101301; RRID: AB_312800 CD4 BD Biosciences #565634; RRID: AB_2739312 CD8 BD Biosciences #565968; RRID: AB_2739421 CD11b BioLegend #101227; RRID: AB_393577 I-A/I-E BD Biosciences #745580; RRID: AB_2743096 B220 BD Biosciences #17045282; RRID:AB_469395 Ly6C BioLegend #128007; RRID: AB_1186133 CD11c BD Biosciences #565451; RRID: AB_2744278 NK1.1 BioLegend #156537; RRID: AB_3083138 F4/80 BioLegend #123107; RRID: AB_893500 Ly6G BD Biosciences #746448; RRID: AB_2743751 CD45 BD Biosciences #557659; RRID: AB_396774 APC-conjugated PD-1 BioLegend #135210; RRID: AB_2159183 Brilliant Violet 421-conjugated KLRG1 BioLegend #138413; RRID:AB_10918627 APC-Cy7-conjugated CD44 BioLegend #103027; RRID: AB_830784 PerCP-Cy5.5-conjugated CD62L BioLegend #104431; RRID:AB_2187123 PE-Cy7-conjugated Sca-1 BioLegend #108113; RRID: AB_493597 FITC-conjugated CD122 BioLegend #123207; RRID: AB_940611 anti-rat CD11b BD Biosciences #550282; RRID: AB_393577 anti-hamster CD31 Millipore #MAB1398Z; RRID: AB_94207 rat anti-mouse F4/80 Bio-Rad #MCA497GA; RRID: AB_323806 rat anti-mouse Ly-6G BioLegend #127602; RRID: AB_1089180 Anti-MLKL (phospho S358) antibody Abcam ab187091; RRID: AB_2619685 Alexa Fluor 488-conjugated goat anti-rat Thermo Fisher Scientific #A11006; RRID: AB_2534074 (Continued on next page) Cell Reports ▪▪, 117226, ▪▪, 2026 17 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Alexa Fluor 568-conjugated goat anti-hamster Iwai Chemicals #127-165-160; RRID: AB_2535761 Alexa Fluor 488-conjugated goat anti-rabbit Thermo Fisher Scientific #A11034; RRID: AB_2576217 PerCP-Cy5.5 anti-CD8 BioLegend #100733; RRID: AB_2075239 Alexa Fluor 647 anti-mouse IFN-γ BioLegend #505816; RRID: AB_493315 anti-mouse PD-L1 Selleck #A2115; RRID: AB_3675704 Bacterial and virus strains DH5α Thermo Fisher Scientific #18258012 Chemicals, peptides, and recombinant proteins DMEM (High Glucose) Nacalai Tesque #08459-64 RPMI 1640 Medium Nacalai Tesque #30264-56 Fetal bovine serum Nissui #174012 7-Cl-O-Nec1 Abcam #ab221984 zVAD-FMK BD Biosciences #305664 Necrostatin-1 Enzo Life Sciences #BML-AP309-0020 Ferrostatin-1 MedChemExpress #HY-100579 cOmpleteTM Protease Inhibitor Cocktail (1×) Roche #11697498001 Chemi-Lumi One Ultra Nacalai Tesque #02230-54 Isogen reagent Nippon Gene #311-02501 PrimeScriptTM reverse transcriptase Takara #RR037A THUNDERBIRD® SYBR® qPCR Mix Toyobo #QPS-201 Sepasol®-RNA I Super G Nacalai Tesque #09379-55 Polybrene Sigma-Aldrich #H9268 4% paraformaldehyde FUJIFILM Wako #163-20145 Acetone Nacalai Tesque #00529-85 Puromycin InvivoGen #ant-pr-1 RBC Lysis Buffer Invitrogen #00-4333-57 Dako Protein Block, Serum-free Agilent #X090930-2 DAPI-Fluoromount-G Southern Biotech #0100-20 Dextran, SNARFTM-1, 70,000 MW, Anionic Invitrogen D3304 Pimo-yne Dr. Sando, University of Tokyo N/A PMX53 Selleck #S6293 Critical commercial assays Calcein AM (Ex/Em = 480/515 nm) Thermo Fisher Scientific #C3099 LDH Cytotoxicity Detection Kit Nacalai Tesque #289-97301 MiSeq v2 Reagent Kit Illumina #MS-102-2002 NucleoSpin Tissue Kit Takara #740952.50 Human Complement C3a ELISA Kit Invitrogen BMS2089 Human Complement C5a ELISA Kit Invitrogen BMS2088 KAPA HiFi HotStart ReadyMix KAPA Biosystems #KK2602 FastGene Gel/PCR Extraction Kit Nippon Genetics #FG-91202 Experimental models: Cell lines .. MIA-PaCa2 ATCC CRL-1420 HeLa ATCC CCL-2 HSML Dr. Kudoh, Hirosaki University, Japan N/A HEK293T ATCC CRL-11268 (Continued on next page) 18 Cell Reports ▪▪, 117226, ▪▪, 2026 Article ll OPEN ACCESS

    Protease Inhibitor:

    Article Title: Tolerance to extracellular acidic pH facilitates tumor plasticity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FAM129C Abcam #ab113755 PIGR Abcam #ab96196; RRID: AB_10677612 β-actin Sigma-Aldrich #A5441; RRID: AB_476744 GPX4 Cell Signaling Technology #52455; RRID: AB_2924984 RIP Cell Signaling Technology #3493; RRID: AB_2305314 phospho-RIP Cell Signaling Technology #65746; RRID: AB_2799693 MLKL Cell Signaling Technology #14993; RRID: AB_2721822 phospho-MLKL Cell Signaling Technology #91689; RRID: AB_2732034 cleaved Caspase-3 Cell Signaling Technology #9661S; RRID: AB_2341188 Caspase-3 Cell Signaling Technology #9662S; RRID: AB_331439 GSDMD Cell Signaling Technology #97558S; RRID: AB_2864253 ZEB1 Cell Signaling Technology #D80D3; RRID: AB_1904164 E-cadherin Cell Signaling Technology #24E10; RRID: AB_2291471 Vimentin Cell Signaling Technology #D21H3; RRID: AB_10695459 Snail Cell Signaling Technology #C15D3; RRID: AB_2255011 C3 Cell Signaling Technology #97425; RRID: AB_2313773 C5a Abcam #202039; RRID: AB_2313773 donkey anti-mouse IgG Sigma-Aldrich #A4416; RRID: AB_2075239 anti-rabbit Cell Signaling Technology #7074P2; RRID: AB_2099233 CD16/32 BioLegend #101301; RRID: AB_312800 CD4 BD Biosciences #565634; RRID: AB_2739312 CD8 BD Biosciences #565968; RRID: AB_2739421 CD11b BioLegend #101227; RRID: AB_393577 I-A/I-E BD Biosciences #745580; RRID: AB_2743096 B220 BD Biosciences #17045282; RRID:AB_469395 Ly6C BioLegend #128007; RRID: AB_1186133 CD11c BD Biosciences #565451; RRID: AB_2744278 NK1.1 BioLegend #156537; RRID: AB_3083138 F4/80 BioLegend #123107; RRID: AB_893500 Ly6G BD Biosciences #746448; RRID: AB_2743751 CD45 BD Biosciences #557659; RRID: AB_396774 APC-conjugated PD-1 BioLegend #135210; RRID: AB_2159183 Brilliant Violet 421-conjugated KLRG1 BioLegend #138413; RRID:AB_10918627 APC-Cy7-conjugated CD44 BioLegend #103027; RRID: AB_830784 PerCP-Cy5.5-conjugated CD62L BioLegend #104431; RRID:AB_2187123 PE-Cy7-conjugated Sca-1 BioLegend #108113; RRID: AB_493597 FITC-conjugated CD122 BioLegend #123207; RRID: AB_940611 anti-rat CD11b BD Biosciences #550282; RRID: AB_393577 anti-hamster CD31 Millipore #MAB1398Z; RRID: AB_94207 rat anti-mouse F4/80 Bio-Rad #MCA497GA; RRID: AB_323806 rat anti-mouse Ly-6G BioLegend #127602; RRID: AB_1089180 Anti-MLKL (phospho S358) antibody Abcam ab187091; RRID: AB_2619685 Alexa Fluor 488-conjugated goat anti-rat Thermo Fisher Scientific #A11006; RRID: AB_2534074 (Continued on next page) Cell Reports ▪▪, 117226, ▪▪, 2026 17 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Alexa Fluor 568-conjugated goat anti-hamster Iwai Chemicals #127-165-160; RRID: AB_2535761 Alexa Fluor 488-conjugated goat anti-rabbit Thermo Fisher Scientific #A11034; RRID: AB_2576217 PerCP-Cy5.5 anti-CD8 BioLegend #100733; RRID: AB_2075239 Alexa Fluor 647 anti-mouse IFN-γ BioLegend #505816; RRID: AB_493315 anti-mouse PD-L1 Selleck #A2115; RRID: AB_3675704 Bacterial and virus strains DH5α Thermo Fisher Scientific #18258012 Chemicals, peptides, and recombinant proteins DMEM (High Glucose) Nacalai Tesque #08459-64 RPMI 1640 Medium Nacalai Tesque #30264-56 Fetal bovine serum Nissui #174012 7-Cl-O-Nec1 Abcam #ab221984 zVAD-FMK BD Biosciences #305664 Necrostatin-1 Enzo Life Sciences #BML-AP309-0020 Ferrostatin-1 MedChemExpress #HY-100579 cOmpleteTM Protease Inhibitor Cocktail (1×) Roche #11697498001 Chemi-Lumi One Ultra Nacalai Tesque #02230-54 Isogen reagent Nippon Gene #311-02501 PrimeScriptTM reverse transcriptase Takara #RR037A THUNDERBIRD® SYBR® qPCR Mix Toyobo #QPS-201 Sepasol®-RNA I Super G Nacalai Tesque #09379-55 Polybrene Sigma-Aldrich #H9268 4% paraformaldehyde FUJIFILM Wako #163-20145 Acetone Nacalai Tesque #00529-85 Puromycin InvivoGen #ant-pr-1 RBC Lysis Buffer Invitrogen #00-4333-57 Dako Protein Block, Serum-free Agilent #X090930-2 DAPI-Fluoromount-G Southern Biotech #0100-20 Dextran, SNARFTM-1, 70,000 MW, Anionic Invitrogen D3304 Pimo-yne Dr. Sando, University of Tokyo N/A PMX53 Selleck #S6293 Critical commercial assays Calcein AM (Ex/Em = 480/515 nm) Thermo Fisher Scientific #C3099 LDH Cytotoxicity Detection Kit Nacalai Tesque #289-97301 MiSeq v2 Reagent Kit Illumina #MS-102-2002 NucleoSpin Tissue Kit Takara #740952.50 Human Complement C3a ELISA Kit Invitrogen BMS2089 Human Complement C5a ELISA Kit Invitrogen BMS2088 KAPA HiFi HotStart ReadyMix KAPA Biosystems #KK2602 FastGene Gel/PCR Extraction Kit Nippon Genetics #FG-91202 Experimental models: Cell lines .. MIA-PaCa2 ATCC CRL-1420 HeLa ATCC CCL-2 HSML Dr. Kudoh, Hirosaki University, Japan N/A HEK293T ATCC CRL-11268 (Continued on next page) 18 Cell Reports ▪▪, 117226, ▪▪, 2026 Article ll OPEN ACCESS

    Reverse Transcription:

    Article Title: Tolerance to extracellular acidic pH facilitates tumor plasticity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FAM129C Abcam #ab113755 PIGR Abcam #ab96196; RRID: AB_10677612 β-actin Sigma-Aldrich #A5441; RRID: AB_476744 GPX4 Cell Signaling Technology #52455; RRID: AB_2924984 RIP Cell Signaling Technology #3493; RRID: AB_2305314 phospho-RIP Cell Signaling Technology #65746; RRID: AB_2799693 MLKL Cell Signaling Technology #14993; RRID: AB_2721822 phospho-MLKL Cell Signaling Technology #91689; RRID: AB_2732034 cleaved Caspase-3 Cell Signaling Technology #9661S; RRID: AB_2341188 Caspase-3 Cell Signaling Technology #9662S; RRID: AB_331439 GSDMD Cell Signaling Technology #97558S; RRID: AB_2864253 ZEB1 Cell Signaling Technology #D80D3; RRID: AB_1904164 E-cadherin Cell Signaling Technology #24E10; RRID: AB_2291471 Vimentin Cell Signaling Technology #D21H3; RRID: AB_10695459 Snail Cell Signaling Technology #C15D3; RRID: AB_2255011 C3 Cell Signaling Technology #97425; RRID: AB_2313773 C5a Abcam #202039; RRID: AB_2313773 donkey anti-mouse IgG Sigma-Aldrich #A4416; RRID: AB_2075239 anti-rabbit Cell Signaling Technology #7074P2; RRID: AB_2099233 CD16/32 BioLegend #101301; RRID: AB_312800 CD4 BD Biosciences #565634; RRID: AB_2739312 CD8 BD Biosciences #565968; RRID: AB_2739421 CD11b BioLegend #101227; RRID: AB_393577 I-A/I-E BD Biosciences #745580; RRID: AB_2743096 B220 BD Biosciences #17045282; RRID:AB_469395 Ly6C BioLegend #128007; RRID: AB_1186133 CD11c BD Biosciences #565451; RRID: AB_2744278 NK1.1 BioLegend #156537; RRID: AB_3083138 F4/80 BioLegend #123107; RRID: AB_893500 Ly6G BD Biosciences #746448; RRID: AB_2743751 CD45 BD Biosciences #557659; RRID: AB_396774 APC-conjugated PD-1 BioLegend #135210; RRID: AB_2159183 Brilliant Violet 421-conjugated KLRG1 BioLegend #138413; RRID:AB_10918627 APC-Cy7-conjugated CD44 BioLegend #103027; RRID: AB_830784 PerCP-Cy5.5-conjugated CD62L BioLegend #104431; RRID:AB_2187123 PE-Cy7-conjugated Sca-1 BioLegend #108113; RRID: AB_493597 FITC-conjugated CD122 BioLegend #123207; RRID: AB_940611 anti-rat CD11b BD Biosciences #550282; RRID: AB_393577 anti-hamster CD31 Millipore #MAB1398Z; RRID: AB_94207 rat anti-mouse F4/80 Bio-Rad #MCA497GA; RRID: AB_323806 rat anti-mouse Ly-6G BioLegend #127602; RRID: AB_1089180 Anti-MLKL (phospho S358) antibody Abcam ab187091; RRID: AB_2619685 Alexa Fluor 488-conjugated goat anti-rat Thermo Fisher Scientific #A11006; RRID: AB_2534074 (Continued on next page) Cell Reports ▪▪, 117226, ▪▪, 2026 17 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Alexa Fluor 568-conjugated goat anti-hamster Iwai Chemicals #127-165-160; RRID: AB_2535761 Alexa Fluor 488-conjugated goat anti-rabbit Thermo Fisher Scientific #A11034; RRID: AB_2576217 PerCP-Cy5.5 anti-CD8 BioLegend #100733; RRID: AB_2075239 Alexa Fluor 647 anti-mouse IFN-γ BioLegend #505816; RRID: AB_493315 anti-mouse PD-L1 Selleck #A2115; RRID: AB_3675704 Bacterial and virus strains DH5α Thermo Fisher Scientific #18258012 Chemicals, peptides, and recombinant proteins DMEM (High Glucose) Nacalai Tesque #08459-64 RPMI 1640 Medium Nacalai Tesque #30264-56 Fetal bovine serum Nissui #174012 7-Cl-O-Nec1 Abcam #ab221984 zVAD-FMK BD Biosciences #305664 Necrostatin-1 Enzo Life Sciences #BML-AP309-0020 Ferrostatin-1 MedChemExpress #HY-100579 cOmpleteTM Protease Inhibitor Cocktail (1×) Roche #11697498001 Chemi-Lumi One Ultra Nacalai Tesque #02230-54 Isogen reagent Nippon Gene #311-02501 PrimeScriptTM reverse transcriptase Takara #RR037A THUNDERBIRD® SYBR® qPCR Mix Toyobo #QPS-201 Sepasol®-RNA I Super G Nacalai Tesque #09379-55 Polybrene Sigma-Aldrich #H9268 4% paraformaldehyde FUJIFILM Wako #163-20145 Acetone Nacalai Tesque #00529-85 Puromycin InvivoGen #ant-pr-1 RBC Lysis Buffer Invitrogen #00-4333-57 Dako Protein Block, Serum-free Agilent #X090930-2 DAPI-Fluoromount-G Southern Biotech #0100-20 Dextran, SNARFTM-1, 70,000 MW, Anionic Invitrogen D3304 Pimo-yne Dr. Sando, University of Tokyo N/A PMX53 Selleck #S6293 Critical commercial assays Calcein AM (Ex/Em = 480/515 nm) Thermo Fisher Scientific #C3099 LDH Cytotoxicity Detection Kit Nacalai Tesque #289-97301 MiSeq v2 Reagent Kit Illumina #MS-102-2002 NucleoSpin Tissue Kit Takara #740952.50 Human Complement C3a ELISA Kit Invitrogen BMS2089 Human Complement C5a ELISA Kit Invitrogen BMS2088 KAPA HiFi HotStart ReadyMix KAPA Biosystems #KK2602 FastGene Gel/PCR Extraction Kit Nippon Genetics #FG-91202 Experimental models: Cell lines .. MIA-PaCa2 ATCC CRL-1420 HeLa ATCC CCL-2 HSML Dr. Kudoh, Hirosaki University, Japan N/A HEK293T ATCC CRL-11268 (Continued on next page) 18 Cell Reports ▪▪, 117226, ▪▪, 2026 Article ll OPEN ACCESS

    Real-time Polymerase Chain Reaction:

    Article Title: Tolerance to extracellular acidic pH facilitates tumor plasticity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FAM129C Abcam #ab113755 PIGR Abcam #ab96196; RRID: AB_10677612 β-actin Sigma-Aldrich #A5441; RRID: AB_476744 GPX4 Cell Signaling Technology #52455; RRID: AB_2924984 RIP Cell Signaling Technology #3493; RRID: AB_2305314 phospho-RIP Cell Signaling Technology #65746; RRID: AB_2799693 MLKL Cell Signaling Technology #14993; RRID: AB_2721822 phospho-MLKL Cell Signaling Technology #91689; RRID: AB_2732034 cleaved Caspase-3 Cell Signaling Technology #9661S; RRID: AB_2341188 Caspase-3 Cell Signaling Technology #9662S; RRID: AB_331439 GSDMD Cell Signaling Technology #97558S; RRID: AB_2864253 ZEB1 Cell Signaling Technology #D80D3; RRID: AB_1904164 E-cadherin Cell Signaling Technology #24E10; RRID: AB_2291471 Vimentin Cell Signaling Technology #D21H3; RRID: AB_10695459 Snail Cell Signaling Technology #C15D3; RRID: AB_2255011 C3 Cell Signaling Technology #97425; RRID: AB_2313773 C5a Abcam #202039; RRID: AB_2313773 donkey anti-mouse IgG Sigma-Aldrich #A4416; RRID: AB_2075239 anti-rabbit Cell Signaling Technology #7074P2; RRID: AB_2099233 CD16/32 BioLegend #101301; RRID: AB_312800 CD4 BD Biosciences #565634; RRID: AB_2739312 CD8 BD Biosciences #565968; RRID: AB_2739421 CD11b BioLegend #101227; RRID: AB_393577 I-A/I-E BD Biosciences #745580; RRID: AB_2743096 B220 BD Biosciences #17045282; RRID:AB_469395 Ly6C BioLegend #128007; RRID: AB_1186133 CD11c BD Biosciences #565451; RRID: AB_2744278 NK1.1 BioLegend #156537; RRID: AB_3083138 F4/80 BioLegend #123107; RRID: AB_893500 Ly6G BD Biosciences #746448; RRID: AB_2743751 CD45 BD Biosciences #557659; RRID: AB_396774 APC-conjugated PD-1 BioLegend #135210; RRID: AB_2159183 Brilliant Violet 421-conjugated KLRG1 BioLegend #138413; RRID:AB_10918627 APC-Cy7-conjugated CD44 BioLegend #103027; RRID: AB_830784 PerCP-Cy5.5-conjugated CD62L BioLegend #104431; RRID:AB_2187123 PE-Cy7-conjugated Sca-1 BioLegend #108113; RRID: AB_493597 FITC-conjugated CD122 BioLegend #123207; RRID: AB_940611 anti-rat CD11b BD Biosciences #550282; RRID: AB_393577 anti-hamster CD31 Millipore #MAB1398Z; RRID: AB_94207 rat anti-mouse F4/80 Bio-Rad #MCA497GA; RRID: AB_323806 rat anti-mouse Ly-6G BioLegend #127602; RRID: AB_1089180 Anti-MLKL (phospho S358) antibody Abcam ab187091; RRID: AB_2619685 Alexa Fluor 488-conjugated goat anti-rat Thermo Fisher Scientific #A11006; RRID: AB_2534074 (Continued on next page) Cell Reports ▪▪, 117226, ▪▪, 2026 17 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Alexa Fluor 568-conjugated goat anti-hamster Iwai Chemicals #127-165-160; RRID: AB_2535761 Alexa Fluor 488-conjugated goat anti-rabbit Thermo Fisher Scientific #A11034; RRID: AB_2576217 PerCP-Cy5.5 anti-CD8 BioLegend #100733; RRID: AB_2075239 Alexa Fluor 647 anti-mouse IFN-γ BioLegend #505816; RRID: AB_493315 anti-mouse PD-L1 Selleck #A2115; RRID: AB_3675704 Bacterial and virus strains DH5α Thermo Fisher Scientific #18258012 Chemicals, peptides, and recombinant proteins DMEM (High Glucose) Nacalai Tesque #08459-64 RPMI 1640 Medium Nacalai Tesque #30264-56 Fetal bovine serum Nissui #174012 7-Cl-O-Nec1 Abcam #ab221984 zVAD-FMK BD Biosciences #305664 Necrostatin-1 Enzo Life Sciences #BML-AP309-0020 Ferrostatin-1 MedChemExpress #HY-100579 cOmpleteTM Protease Inhibitor Cocktail (1×) Roche #11697498001 Chemi-Lumi One Ultra Nacalai Tesque #02230-54 Isogen reagent Nippon Gene #311-02501 PrimeScriptTM reverse transcriptase Takara #RR037A THUNDERBIRD® SYBR® qPCR Mix Toyobo #QPS-201 Sepasol®-RNA I Super G Nacalai Tesque #09379-55 Polybrene Sigma-Aldrich #H9268 4% paraformaldehyde FUJIFILM Wako #163-20145 Acetone Nacalai Tesque #00529-85 Puromycin InvivoGen #ant-pr-1 RBC Lysis Buffer Invitrogen #00-4333-57 Dako Protein Block, Serum-free Agilent #X090930-2 DAPI-Fluoromount-G Southern Biotech #0100-20 Dextran, SNARFTM-1, 70,000 MW, Anionic Invitrogen D3304 Pimo-yne Dr. Sando, University of Tokyo N/A PMX53 Selleck #S6293 Critical commercial assays Calcein AM (Ex/Em = 480/515 nm) Thermo Fisher Scientific #C3099 LDH Cytotoxicity Detection Kit Nacalai Tesque #289-97301 MiSeq v2 Reagent Kit Illumina #MS-102-2002 NucleoSpin Tissue Kit Takara #740952.50 Human Complement C3a ELISA Kit Invitrogen BMS2089 Human Complement C5a ELISA Kit Invitrogen BMS2088 KAPA HiFi HotStart ReadyMix KAPA Biosystems #KK2602 FastGene Gel/PCR Extraction Kit Nippon Genetics #FG-91202 Experimental models: Cell lines .. MIA-PaCa2 ATCC CRL-1420 HeLa ATCC CCL-2 HSML Dr. Kudoh, Hirosaki University, Japan N/A HEK293T ATCC CRL-11268 (Continued on next page) 18 Cell Reports ▪▪, 117226, ▪▪, 2026 Article ll OPEN ACCESS

    Red Blood Cell Lysis:

    Article Title: Tolerance to extracellular acidic pH facilitates tumor plasticity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FAM129C Abcam #ab113755 PIGR Abcam #ab96196; RRID: AB_10677612 β-actin Sigma-Aldrich #A5441; RRID: AB_476744 GPX4 Cell Signaling Technology #52455; RRID: AB_2924984 RIP Cell Signaling Technology #3493; RRID: AB_2305314 phospho-RIP Cell Signaling Technology #65746; RRID: AB_2799693 MLKL Cell Signaling Technology #14993; RRID: AB_2721822 phospho-MLKL Cell Signaling Technology #91689; RRID: AB_2732034 cleaved Caspase-3 Cell Signaling Technology #9661S; RRID: AB_2341188 Caspase-3 Cell Signaling Technology #9662S; RRID: AB_331439 GSDMD Cell Signaling Technology #97558S; RRID: AB_2864253 ZEB1 Cell Signaling Technology #D80D3; RRID: AB_1904164 E-cadherin Cell Signaling Technology #24E10; RRID: AB_2291471 Vimentin Cell Signaling Technology #D21H3; RRID: AB_10695459 Snail Cell Signaling Technology #C15D3; RRID: AB_2255011 C3 Cell Signaling Technology #97425; RRID: AB_2313773 C5a Abcam #202039; RRID: AB_2313773 donkey anti-mouse IgG Sigma-Aldrich #A4416; RRID: AB_2075239 anti-rabbit Cell Signaling Technology #7074P2; RRID: AB_2099233 CD16/32 BioLegend #101301; RRID: AB_312800 CD4 BD Biosciences #565634; RRID: AB_2739312 CD8 BD Biosciences #565968; RRID: AB_2739421 CD11b BioLegend #101227; RRID: AB_393577 I-A/I-E BD Biosciences #745580; RRID: AB_2743096 B220 BD Biosciences #17045282; RRID:AB_469395 Ly6C BioLegend #128007; RRID: AB_1186133 CD11c BD Biosciences #565451; RRID: AB_2744278 NK1.1 BioLegend #156537; RRID: AB_3083138 F4/80 BioLegend #123107; RRID: AB_893500 Ly6G BD Biosciences #746448; RRID: AB_2743751 CD45 BD Biosciences #557659; RRID: AB_396774 APC-conjugated PD-1 BioLegend #135210; RRID: AB_2159183 Brilliant Violet 421-conjugated KLRG1 BioLegend #138413; RRID:AB_10918627 APC-Cy7-conjugated CD44 BioLegend #103027; RRID: AB_830784 PerCP-Cy5.5-conjugated CD62L BioLegend #104431; RRID:AB_2187123 PE-Cy7-conjugated Sca-1 BioLegend #108113; RRID: AB_493597 FITC-conjugated CD122 BioLegend #123207; RRID: AB_940611 anti-rat CD11b BD Biosciences #550282; RRID: AB_393577 anti-hamster CD31 Millipore #MAB1398Z; RRID: AB_94207 rat anti-mouse F4/80 Bio-Rad #MCA497GA; RRID: AB_323806 rat anti-mouse Ly-6G BioLegend #127602; RRID: AB_1089180 Anti-MLKL (phospho S358) antibody Abcam ab187091; RRID: AB_2619685 Alexa Fluor 488-conjugated goat anti-rat Thermo Fisher Scientific #A11006; RRID: AB_2534074 (Continued on next page) Cell Reports ▪▪, 117226, ▪▪, 2026 17 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Alexa Fluor 568-conjugated goat anti-hamster Iwai Chemicals #127-165-160; RRID: AB_2535761 Alexa Fluor 488-conjugated goat anti-rabbit Thermo Fisher Scientific #A11034; RRID: AB_2576217 PerCP-Cy5.5 anti-CD8 BioLegend #100733; RRID: AB_2075239 Alexa Fluor 647 anti-mouse IFN-γ BioLegend #505816; RRID: AB_493315 anti-mouse PD-L1 Selleck #A2115; RRID: AB_3675704 Bacterial and virus strains DH5α Thermo Fisher Scientific #18258012 Chemicals, peptides, and recombinant proteins DMEM (High Glucose) Nacalai Tesque #08459-64 RPMI 1640 Medium Nacalai Tesque #30264-56 Fetal bovine serum Nissui #174012 7-Cl-O-Nec1 Abcam #ab221984 zVAD-FMK BD Biosciences #305664 Necrostatin-1 Enzo Life Sciences #BML-AP309-0020 Ferrostatin-1 MedChemExpress #HY-100579 cOmpleteTM Protease Inhibitor Cocktail (1×) Roche #11697498001 Chemi-Lumi One Ultra Nacalai Tesque #02230-54 Isogen reagent Nippon Gene #311-02501 PrimeScriptTM reverse transcriptase Takara #RR037A THUNDERBIRD® SYBR® qPCR Mix Toyobo #QPS-201 Sepasol®-RNA I Super G Nacalai Tesque #09379-55 Polybrene Sigma-Aldrich #H9268 4% paraformaldehyde FUJIFILM Wako #163-20145 Acetone Nacalai Tesque #00529-85 Puromycin InvivoGen #ant-pr-1 RBC Lysis Buffer Invitrogen #00-4333-57 Dako Protein Block, Serum-free Agilent #X090930-2 DAPI-Fluoromount-G Southern Biotech #0100-20 Dextran, SNARFTM-1, 70,000 MW, Anionic Invitrogen D3304 Pimo-yne Dr. Sando, University of Tokyo N/A PMX53 Selleck #S6293 Critical commercial assays Calcein AM (Ex/Em = 480/515 nm) Thermo Fisher Scientific #C3099 LDH Cytotoxicity Detection Kit Nacalai Tesque #289-97301 MiSeq v2 Reagent Kit Illumina #MS-102-2002 NucleoSpin Tissue Kit Takara #740952.50 Human Complement C3a ELISA Kit Invitrogen BMS2089 Human Complement C5a ELISA Kit Invitrogen BMS2088 KAPA HiFi HotStart ReadyMix KAPA Biosystems #KK2602 FastGene Gel/PCR Extraction Kit Nippon Genetics #FG-91202 Experimental models: Cell lines .. MIA-PaCa2 ATCC CRL-1420 HeLa ATCC CCL-2 HSML Dr. Kudoh, Hirosaki University, Japan N/A HEK293T ATCC CRL-11268 (Continued on next page) 18 Cell Reports ▪▪, 117226, ▪▪, 2026 Article ll OPEN ACCESS

    Blocking Assay:

    Article Title: Tolerance to extracellular acidic pH facilitates tumor plasticity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FAM129C Abcam #ab113755 PIGR Abcam #ab96196; RRID: AB_10677612 β-actin Sigma-Aldrich #A5441; RRID: AB_476744 GPX4 Cell Signaling Technology #52455; RRID: AB_2924984 RIP Cell Signaling Technology #3493; RRID: AB_2305314 phospho-RIP Cell Signaling Technology #65746; RRID: AB_2799693 MLKL Cell Signaling Technology #14993; RRID: AB_2721822 phospho-MLKL Cell Signaling Technology #91689; RRID: AB_2732034 cleaved Caspase-3 Cell Signaling Technology #9661S; RRID: AB_2341188 Caspase-3 Cell Signaling Technology #9662S; RRID: AB_331439 GSDMD Cell Signaling Technology #97558S; RRID: AB_2864253 ZEB1 Cell Signaling Technology #D80D3; RRID: AB_1904164 E-cadherin Cell Signaling Technology #24E10; RRID: AB_2291471 Vimentin Cell Signaling Technology #D21H3; RRID: AB_10695459 Snail Cell Signaling Technology #C15D3; RRID: AB_2255011 C3 Cell Signaling Technology #97425; RRID: AB_2313773 C5a Abcam #202039; RRID: AB_2313773 donkey anti-mouse IgG Sigma-Aldrich #A4416; RRID: AB_2075239 anti-rabbit Cell Signaling Technology #7074P2; RRID: AB_2099233 CD16/32 BioLegend #101301; RRID: AB_312800 CD4 BD Biosciences #565634; RRID: AB_2739312 CD8 BD Biosciences #565968; RRID: AB_2739421 CD11b BioLegend #101227; RRID: AB_393577 I-A/I-E BD Biosciences #745580; RRID: AB_2743096 B220 BD Biosciences #17045282; RRID:AB_469395 Ly6C BioLegend #128007; RRID: AB_1186133 CD11c BD Biosciences #565451; RRID: AB_2744278 NK1.1 BioLegend #156537; RRID: AB_3083138 F4/80 BioLegend #123107; RRID: AB_893500 Ly6G BD Biosciences #746448; RRID: AB_2743751 CD45 BD Biosciences #557659; RRID: AB_396774 APC-conjugated PD-1 BioLegend #135210; RRID: AB_2159183 Brilliant Violet 421-conjugated KLRG1 BioLegend #138413; RRID:AB_10918627 APC-Cy7-conjugated CD44 BioLegend #103027; RRID: AB_830784 PerCP-Cy5.5-conjugated CD62L BioLegend #104431; RRID:AB_2187123 PE-Cy7-conjugated Sca-1 BioLegend #108113; RRID: AB_493597 FITC-conjugated CD122 BioLegend #123207; RRID: AB_940611 anti-rat CD11b BD Biosciences #550282; RRID: AB_393577 anti-hamster CD31 Millipore #MAB1398Z; RRID: AB_94207 rat anti-mouse F4/80 Bio-Rad #MCA497GA; RRID: AB_323806 rat anti-mouse Ly-6G BioLegend #127602; RRID: AB_1089180 Anti-MLKL (phospho S358) antibody Abcam ab187091; RRID: AB_2619685 Alexa Fluor 488-conjugated goat anti-rat Thermo Fisher Scientific #A11006; RRID: AB_2534074 (Continued on next page) Cell Reports ▪▪, 117226, ▪▪, 2026 17 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Alexa Fluor 568-conjugated goat anti-hamster Iwai Chemicals #127-165-160; RRID: AB_2535761 Alexa Fluor 488-conjugated goat anti-rabbit Thermo Fisher Scientific #A11034; RRID: AB_2576217 PerCP-Cy5.5 anti-CD8 BioLegend #100733; RRID: AB_2075239 Alexa Fluor 647 anti-mouse IFN-γ BioLegend #505816; RRID: AB_493315 anti-mouse PD-L1 Selleck #A2115; RRID: AB_3675704 Bacterial and virus strains DH5α Thermo Fisher Scientific #18258012 Chemicals, peptides, and recombinant proteins DMEM (High Glucose) Nacalai Tesque #08459-64 RPMI 1640 Medium Nacalai Tesque #30264-56 Fetal bovine serum Nissui #174012 7-Cl-O-Nec1 Abcam #ab221984 zVAD-FMK BD Biosciences #305664 Necrostatin-1 Enzo Life Sciences #BML-AP309-0020 Ferrostatin-1 MedChemExpress #HY-100579 cOmpleteTM Protease Inhibitor Cocktail (1×) Roche #11697498001 Chemi-Lumi One Ultra Nacalai Tesque #02230-54 Isogen reagent Nippon Gene #311-02501 PrimeScriptTM reverse transcriptase Takara #RR037A THUNDERBIRD® SYBR® qPCR Mix Toyobo #QPS-201 Sepasol®-RNA I Super G Nacalai Tesque #09379-55 Polybrene Sigma-Aldrich #H9268 4% paraformaldehyde FUJIFILM Wako #163-20145 Acetone Nacalai Tesque #00529-85 Puromycin InvivoGen #ant-pr-1 RBC Lysis Buffer Invitrogen #00-4333-57 Dako Protein Block, Serum-free Agilent #X090930-2 DAPI-Fluoromount-G Southern Biotech #0100-20 Dextran, SNARFTM-1, 70,000 MW, Anionic Invitrogen D3304 Pimo-yne Dr. Sando, University of Tokyo N/A PMX53 Selleck #S6293 Critical commercial assays Calcein AM (Ex/Em = 480/515 nm) Thermo Fisher Scientific #C3099 LDH Cytotoxicity Detection Kit Nacalai Tesque #289-97301 MiSeq v2 Reagent Kit Illumina #MS-102-2002 NucleoSpin Tissue Kit Takara #740952.50 Human Complement C3a ELISA Kit Invitrogen BMS2089 Human Complement C5a ELISA Kit Invitrogen BMS2088 KAPA HiFi HotStart ReadyMix KAPA Biosystems #KK2602 FastGene Gel/PCR Extraction Kit Nippon Genetics #FG-91202 Experimental models: Cell lines .. MIA-PaCa2 ATCC CRL-1420 HeLa ATCC CCL-2 HSML Dr. Kudoh, Hirosaki University, Japan N/A HEK293T ATCC CRL-11268 (Continued on next page) 18 Cell Reports ▪▪, 117226, ▪▪, 2026 Article ll OPEN ACCESS

    Enzyme-linked Immunosorbent Assay:

    Article Title: Tolerance to extracellular acidic pH facilitates tumor plasticity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FAM129C Abcam #ab113755 PIGR Abcam #ab96196; RRID: AB_10677612 β-actin Sigma-Aldrich #A5441; RRID: AB_476744 GPX4 Cell Signaling Technology #52455; RRID: AB_2924984 RIP Cell Signaling Technology #3493; RRID: AB_2305314 phospho-RIP Cell Signaling Technology #65746; RRID: AB_2799693 MLKL Cell Signaling Technology #14993; RRID: AB_2721822 phospho-MLKL Cell Signaling Technology #91689; RRID: AB_2732034 cleaved Caspase-3 Cell Signaling Technology #9661S; RRID: AB_2341188 Caspase-3 Cell Signaling Technology #9662S; RRID: AB_331439 GSDMD Cell Signaling Technology #97558S; RRID: AB_2864253 ZEB1 Cell Signaling Technology #D80D3; RRID: AB_1904164 E-cadherin Cell Signaling Technology #24E10; RRID: AB_2291471 Vimentin Cell Signaling Technology #D21H3; RRID: AB_10695459 Snail Cell Signaling Technology #C15D3; RRID: AB_2255011 C3 Cell Signaling Technology #97425; RRID: AB_2313773 C5a Abcam #202039; RRID: AB_2313773 donkey anti-mouse IgG Sigma-Aldrich #A4416; RRID: AB_2075239 anti-rabbit Cell Signaling Technology #7074P2; RRID: AB_2099233 CD16/32 BioLegend #101301; RRID: AB_312800 CD4 BD Biosciences #565634; RRID: AB_2739312 CD8 BD Biosciences #565968; RRID: AB_2739421 CD11b BioLegend #101227; RRID: AB_393577 I-A/I-E BD Biosciences #745580; RRID: AB_2743096 B220 BD Biosciences #17045282; RRID:AB_469395 Ly6C BioLegend #128007; RRID: AB_1186133 CD11c BD Biosciences #565451; RRID: AB_2744278 NK1.1 BioLegend #156537; RRID: AB_3083138 F4/80 BioLegend #123107; RRID: AB_893500 Ly6G BD Biosciences #746448; RRID: AB_2743751 CD45 BD Biosciences #557659; RRID: AB_396774 APC-conjugated PD-1 BioLegend #135210; RRID: AB_2159183 Brilliant Violet 421-conjugated KLRG1 BioLegend #138413; RRID:AB_10918627 APC-Cy7-conjugated CD44 BioLegend #103027; RRID: AB_830784 PerCP-Cy5.5-conjugated CD62L BioLegend #104431; RRID:AB_2187123 PE-Cy7-conjugated Sca-1 BioLegend #108113; RRID: AB_493597 FITC-conjugated CD122 BioLegend #123207; RRID: AB_940611 anti-rat CD11b BD Biosciences #550282; RRID: AB_393577 anti-hamster CD31 Millipore #MAB1398Z; RRID: AB_94207 rat anti-mouse F4/80 Bio-Rad #MCA497GA; RRID: AB_323806 rat anti-mouse Ly-6G BioLegend #127602; RRID: AB_1089180 Anti-MLKL (phospho S358) antibody Abcam ab187091; RRID: AB_2619685 Alexa Fluor 488-conjugated goat anti-rat Thermo Fisher Scientific #A11006; RRID: AB_2534074 (Continued on next page) Cell Reports ▪▪, 117226, ▪▪, 2026 17 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Alexa Fluor 568-conjugated goat anti-hamster Iwai Chemicals #127-165-160; RRID: AB_2535761 Alexa Fluor 488-conjugated goat anti-rabbit Thermo Fisher Scientific #A11034; RRID: AB_2576217 PerCP-Cy5.5 anti-CD8 BioLegend #100733; RRID: AB_2075239 Alexa Fluor 647 anti-mouse IFN-γ BioLegend #505816; RRID: AB_493315 anti-mouse PD-L1 Selleck #A2115; RRID: AB_3675704 Bacterial and virus strains DH5α Thermo Fisher Scientific #18258012 Chemicals, peptides, and recombinant proteins DMEM (High Glucose) Nacalai Tesque #08459-64 RPMI 1640 Medium Nacalai Tesque #30264-56 Fetal bovine serum Nissui #174012 7-Cl-O-Nec1 Abcam #ab221984 zVAD-FMK BD Biosciences #305664 Necrostatin-1 Enzo Life Sciences #BML-AP309-0020 Ferrostatin-1 MedChemExpress #HY-100579 cOmpleteTM Protease Inhibitor Cocktail (1×) Roche #11697498001 Chemi-Lumi One Ultra Nacalai Tesque #02230-54 Isogen reagent Nippon Gene #311-02501 PrimeScriptTM reverse transcriptase Takara #RR037A THUNDERBIRD® SYBR® qPCR Mix Toyobo #QPS-201 Sepasol®-RNA I Super G Nacalai Tesque #09379-55 Polybrene Sigma-Aldrich #H9268 4% paraformaldehyde FUJIFILM Wako #163-20145 Acetone Nacalai Tesque #00529-85 Puromycin InvivoGen #ant-pr-1 RBC Lysis Buffer Invitrogen #00-4333-57 Dako Protein Block, Serum-free Agilent #X090930-2 DAPI-Fluoromount-G Southern Biotech #0100-20 Dextran, SNARFTM-1, 70,000 MW, Anionic Invitrogen D3304 Pimo-yne Dr. Sando, University of Tokyo N/A PMX53 Selleck #S6293 Critical commercial assays Calcein AM (Ex/Em = 480/515 nm) Thermo Fisher Scientific #C3099 LDH Cytotoxicity Detection Kit Nacalai Tesque #289-97301 MiSeq v2 Reagent Kit Illumina #MS-102-2002 NucleoSpin Tissue Kit Takara #740952.50 Human Complement C3a ELISA Kit Invitrogen BMS2089 Human Complement C5a ELISA Kit Invitrogen BMS2088 KAPA HiFi HotStart ReadyMix KAPA Biosystems #KK2602 FastGene Gel/PCR Extraction Kit Nippon Genetics #FG-91202 Experimental models: Cell lines .. MIA-PaCa2 ATCC CRL-1420 HeLa ATCC CCL-2 HSML Dr. Kudoh, Hirosaki University, Japan N/A HEK293T ATCC CRL-11268 (Continued on next page) 18 Cell Reports ▪▪, 117226, ▪▪, 2026 Article ll OPEN ACCESS

    Extraction:

    Article Title: Tolerance to extracellular acidic pH facilitates tumor plasticity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FAM129C Abcam #ab113755 PIGR Abcam #ab96196; RRID: AB_10677612 β-actin Sigma-Aldrich #A5441; RRID: AB_476744 GPX4 Cell Signaling Technology #52455; RRID: AB_2924984 RIP Cell Signaling Technology #3493; RRID: AB_2305314 phospho-RIP Cell Signaling Technology #65746; RRID: AB_2799693 MLKL Cell Signaling Technology #14993; RRID: AB_2721822 phospho-MLKL Cell Signaling Technology #91689; RRID: AB_2732034 cleaved Caspase-3 Cell Signaling Technology #9661S; RRID: AB_2341188 Caspase-3 Cell Signaling Technology #9662S; RRID: AB_331439 GSDMD Cell Signaling Technology #97558S; RRID: AB_2864253 ZEB1 Cell Signaling Technology #D80D3; RRID: AB_1904164 E-cadherin Cell Signaling Technology #24E10; RRID: AB_2291471 Vimentin Cell Signaling Technology #D21H3; RRID: AB_10695459 Snail Cell Signaling Technology #C15D3; RRID: AB_2255011 C3 Cell Signaling Technology #97425; RRID: AB_2313773 C5a Abcam #202039; RRID: AB_2313773 donkey anti-mouse IgG Sigma-Aldrich #A4416; RRID: AB_2075239 anti-rabbit Cell Signaling Technology #7074P2; RRID: AB_2099233 CD16/32 BioLegend #101301; RRID: AB_312800 CD4 BD Biosciences #565634; RRID: AB_2739312 CD8 BD Biosciences #565968; RRID: AB_2739421 CD11b BioLegend #101227; RRID: AB_393577 I-A/I-E BD Biosciences #745580; RRID: AB_2743096 B220 BD Biosciences #17045282; RRID:AB_469395 Ly6C BioLegend #128007; RRID: AB_1186133 CD11c BD Biosciences #565451; RRID: AB_2744278 NK1.1 BioLegend #156537; RRID: AB_3083138 F4/80 BioLegend #123107; RRID: AB_893500 Ly6G BD Biosciences #746448; RRID: AB_2743751 CD45 BD Biosciences #557659; RRID: AB_396774 APC-conjugated PD-1 BioLegend #135210; RRID: AB_2159183 Brilliant Violet 421-conjugated KLRG1 BioLegend #138413; RRID:AB_10918627 APC-Cy7-conjugated CD44 BioLegend #103027; RRID: AB_830784 PerCP-Cy5.5-conjugated CD62L BioLegend #104431; RRID:AB_2187123 PE-Cy7-conjugated Sca-1 BioLegend #108113; RRID: AB_493597 FITC-conjugated CD122 BioLegend #123207; RRID: AB_940611 anti-rat CD11b BD Biosciences #550282; RRID: AB_393577 anti-hamster CD31 Millipore #MAB1398Z; RRID: AB_94207 rat anti-mouse F4/80 Bio-Rad #MCA497GA; RRID: AB_323806 rat anti-mouse Ly-6G BioLegend #127602; RRID: AB_1089180 Anti-MLKL (phospho S358) antibody Abcam ab187091; RRID: AB_2619685 Alexa Fluor 488-conjugated goat anti-rat Thermo Fisher Scientific #A11006; RRID: AB_2534074 (Continued on next page) Cell Reports ▪▪, 117226, ▪▪, 2026 17 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Alexa Fluor 568-conjugated goat anti-hamster Iwai Chemicals #127-165-160; RRID: AB_2535761 Alexa Fluor 488-conjugated goat anti-rabbit Thermo Fisher Scientific #A11034; RRID: AB_2576217 PerCP-Cy5.5 anti-CD8 BioLegend #100733; RRID: AB_2075239 Alexa Fluor 647 anti-mouse IFN-γ BioLegend #505816; RRID: AB_493315 anti-mouse PD-L1 Selleck #A2115; RRID: AB_3675704 Bacterial and virus strains DH5α Thermo Fisher Scientific #18258012 Chemicals, peptides, and recombinant proteins DMEM (High Glucose) Nacalai Tesque #08459-64 RPMI 1640 Medium Nacalai Tesque #30264-56 Fetal bovine serum Nissui #174012 7-Cl-O-Nec1 Abcam #ab221984 zVAD-FMK BD Biosciences #305664 Necrostatin-1 Enzo Life Sciences #BML-AP309-0020 Ferrostatin-1 MedChemExpress #HY-100579 cOmpleteTM Protease Inhibitor Cocktail (1×) Roche #11697498001 Chemi-Lumi One Ultra Nacalai Tesque #02230-54 Isogen reagent Nippon Gene #311-02501 PrimeScriptTM reverse transcriptase Takara #RR037A THUNDERBIRD® SYBR® qPCR Mix Toyobo #QPS-201 Sepasol®-RNA I Super G Nacalai Tesque #09379-55 Polybrene Sigma-Aldrich #H9268 4% paraformaldehyde FUJIFILM Wako #163-20145 Acetone Nacalai Tesque #00529-85 Puromycin InvivoGen #ant-pr-1 RBC Lysis Buffer Invitrogen #00-4333-57 Dako Protein Block, Serum-free Agilent #X090930-2 DAPI-Fluoromount-G Southern Biotech #0100-20 Dextran, SNARFTM-1, 70,000 MW, Anionic Invitrogen D3304 Pimo-yne Dr. Sando, University of Tokyo N/A PMX53 Selleck #S6293 Critical commercial assays Calcein AM (Ex/Em = 480/515 nm) Thermo Fisher Scientific #C3099 LDH Cytotoxicity Detection Kit Nacalai Tesque #289-97301 MiSeq v2 Reagent Kit Illumina #MS-102-2002 NucleoSpin Tissue Kit Takara #740952.50 Human Complement C3a ELISA Kit Invitrogen BMS2089 Human Complement C5a ELISA Kit Invitrogen BMS2088 KAPA HiFi HotStart ReadyMix KAPA Biosystems #KK2602 FastGene Gel/PCR Extraction Kit Nippon Genetics #FG-91202 Experimental models: Cell lines .. MIA-PaCa2 ATCC CRL-1420 HeLa ATCC CCL-2 HSML Dr. Kudoh, Hirosaki University, Japan N/A HEK293T ATCC CRL-11268 (Continued on next page) 18 Cell Reports ▪▪, 117226, ▪▪, 2026 Article ll OPEN ACCESS



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